|
KMID : 1039420180520030164
|
|
Journal of Pathology and Translational Medicine
2018 Volume.52 No. 3 p.164 ~ p.170
|
|
|
The Major Role of NF-κB in the Depth of Invasion on Acral Melanoma by Decreasing CD8+ T Cells
|
|
Usman Hermin Aminah
Hernowo Bethy S.
Tobing Maringan Diapari Lumban
Hindritiani Reti
|
|
|
Abstract
|
|
|
Background: The tumor microenvironment including immune surveillance affects malignant melanoma (MM) behavior. Nuclear factor κB (NF-κB) stimulates the transcription of various genes in the nucleus and plays a role in the inflammatory process and in tumorigenesis. CD8+ T cells have cytotoxic properties important in the elimination of tumors. However, inhibitory receptors on the cell surface will bind to programmed death-ligand 1 (PD-L1), causing CD8+ T cells to lose their ability to initiate an immune response. This study analyzed the association of NF-κB and PD-L1 expression levels and CD8+ T-cell counts with depth of invasion of acral MM, which may be a predictor of aggressiveness related to an increased risk of metastasis.
Methods: A retrospective cross-sectional study was conducted in the Department of Anatomical Pathology, Faculty of Medicine, Universitas Padjadjaran/Hasan Sadikin Hospital using 96 cases of acral melanoma. Immunohistochemical staining was performed on paraffin blocks using anti?NF-κB, ?PD-L1, and -CD8 antibodies and invasion depth was measured using dotSlide-imaging software.
Results: The study showed significant associations between the individual expression of NF-κB and PD-L1 and CD8+ T-cell number, with MM invasion depth. NF-κB was found to be a confounding variable of CD8+ T-cell number (p < .05), but not for PD-L1 expression (p = .154). Through multivariate analysis it was found that NF-κB had the greatest association with the depth of invasion (p < .001), whereas PD-L1 was unrelated to the depth of invasion because it depends on the number of CD8+ T cells (p = .870).
Conclusions: NF-κB plays a major role in acral MM invasion, by decreasing the number of CD8+ T cells in acral MM.
|
|
|
KEYWORD
|
|
|
Acral, CD8+ T cells, Invasion, Melanoma, NF-κB, PD-L1
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|